Coding
Part:BBa_K4044003:Design
Designed by: Kalinichenko Andrei Group: iGEM21_LMSU (2021-10-19)
BcLOV4
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 354
Illegal PstI site found at 598 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 354
Illegal PstI site found at 598 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 354
Illegal PstI site found at 598 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 354
Illegal PstI site found at 598 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization of the nucleotide sequence for efficient gene expression in E. coli was performed using GENEWIZ codon optimization tool.
Source
BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli.
Source organism: Botrytis cinerea